B AC K G R O U N D Propolis and honey samples have been used in folk medicine since ancient times. The antimicrobial activity of six-ethanol extract of propolis (EEP) and five honey samples against Escherichia coli, Staphylococcus aureus, Acinetobacter lwoffii, Pseudomonas aeruginosa a n d Candida albicans was evaluated by macrodilution broth method as described by CLSI. M ET H O D S All reference strains were susceptible and the min imal inhib itory conc e ntration min imum inh ib itory conc e ntration (MIC) val ues ranged from 3.7 to 281.2 μg/ml for EEP and from 15.6 to 500 μg/ml for honey samples. Propolis s a m ples (mean MIC val ue: 77.2±104.2) had higher ant ibact er ial act ivity against all tested strains when comp ared with honey (mean MIC val ue: 170.6±140.1). Also, this difference was found statistically significant (p=0.001). R E S U LT S While Cumakizik-Bursa EEP showed most effective to reference strains totally when compared with other EEP (mean MIC value: 17.2 μg/ml). Anzer Rize-4 honey showed most effective to reference strains totally when compared with other honey samples (mean MIC value: 68.5 μg/ml). CO N C L U S I O N S It was concluded that Turkish EEP and honey samples had in vitro antimicrobial effects against all tested strains including bacteria and fungi in this study.