B AC K G R O U N D While treating patients in neonatal intensive care unit (NICU), bacterial flora and determining antibiotic susceptibility profiles have great importance. M ET H O D S C reactive protein, peripheral smear, CBC and blood culture were used as laboratory investigations. At the same time, urine, lesion and drain cultures, lumbar puncture, chest X-ray and other radiological investigations were made if necessary. R E S U LT S Fifty- seven (24.5%) procreation have been found among 232 cultures. Mostly K. pneumonia 22.8%, coagulase negative Staphylococcus aureus (CONS) 22.8% and Escherichia coli were isolated. K. pneumonia and CONS were mostly isolated organisms of blood cultures and E. coli from urine cultures. Methicillin resistance rate of Staphylococcus aureus was 37.5%. CO N C L U S I O N S CONS and S. aur eu s as a gram-posit ive agent and K. pneumoniae and E. coli as a gram-negative agent were f ound as the most freq uently isolated micr oorganisms in our neo n atal intensive care unit. Beside carbapenems, amikacin and third gen erat ion ceph alosporins were also very highly sensitive against gram-neg at ive bacteria.